同型半胱氨酸（酶循环法）
同型半胱氨酸（酶循环法）
General Assay Characteristics
Assay Principle
CBS
丝氨酸 + 同型半胱氨酸 ———→ L-胱硫醚
CBL
L-胱硫醚———→同型半胱氨酸 ＋ 丙酮酸 + NH3
LDH
丙酮酸 + NADH+ ———→ 乳酸 ＋ NAD +
Proposed Hcy Recipe
Reagent I
Reagent II
Major reagent ingredients made by Nodelbio are list on right
Standard&Control
Home Made: DL-homocysteine in HCl
Commercial available: Bio-Rad: 10.8±2.18, Cat# 34982; 28.5±5.6 Cat# 34982; BioTech homocysteine calibrator(SFDA Registration # 2013-2400218)
Reaction curve chart
Calibrator 1：0.0 μmol/L Calibrator 2：28.0 μmol/L
General Assay Characteristics
| Method | Enzymatic/RATE A/E |
| Reagents | Ready to use |
| Calibrators | Level 1-0.0 μmol/L; Level 2-28.0 μmol/L |
| Calibration Stability | 28 days |
| Sample type | EDTA,SST,Serum,Heparinised plasma |
| Assay range | 1-50.0 μmol/L undiluted |
| Standardisation | NIST SRM 1955”Homocysteine Standard Reference Material” |
CBS
丝氨酸 + 同型半胱氨酸 ———→ L-胱硫醚
CBL
L-胱硫醚———→同型半胱氨酸 ＋ 丙酮酸 + NH3
LDH
丙酮酸 + NADH+ ———→ 乳酸 ＋ NAD +
Proposed Hcy Recipe
Reagent I
| Composition | Concentration | Catalog Number |
| Tris-HCl buffer | 50mM | |
| NADH | 0.45mM | CS001 |
| Serine | 0.5mM | |
| TCEP | 2.0mM | |
| LDH | 10KU | EM005 |
| Detergent, Preservative |
| Composition | Concentration | Catalog Number |
| K-phosphate, Ph7.5 | 200mM | |
| CBS | 0.45mM | EM002 |
| CBL | 0.5mM | EM003 |
| EDTA | 2.5mM | |
| Tritoon X-100 | 0.05% | |
| Detergent, Preservative |
Standard&Control
Home Made: DL-homocysteine in HCl
Commercial available: Bio-Rad: 10.8±2.18, Cat# 34982; 28.5±5.6 Cat# 34982; BioTech homocysteine calibrator(SFDA Registration # 2013-2400218)
Reaction curve chart
Calibrator 1：0.0 μmol/L Calibrator 2：28.0 μmol/L